The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
Why Choose VeloGene Biotechnology?
What Is a Conditional Knockout Mouse Model via Tetraploid Complementation?
VeloGene Biotechnology Condition Knockout(cKO) Mouse Model Customizing Service
F0 "Founder" Cre⁺fl/fl Ready - to -Use
In neuroscience, immunology, metabolism, and oncology research, conditional knockout (CKO) mice are essential tools for dissecting tissue-specific gene functions. Traditional workflows require separately generating Flox mice and Cre driver mice, followed by multiple generations of breeding—taking over a year—while multi-locus editing on the same chromosome is nearly impossible.
VeloGene Biotechnology’s TurboMice™ tetraploid complementation technology completes the introduction of loxP sites + integration of Cre elements at the embryonic stem cell (ESC) stage in a single step. Through tetraploid embryo complementation, 100% of the F0 generation directly yields Cre-positive + Flox homozygous mice(Genefl/fl, Cre+)derived entirely from edited stem cells—bypassing chimeras, skipping breeding generations, and eliminating guesswork-style mating.
What are "Conditional Knockout Mice" under the TurboMice™ Tetraploid Complementation Route?
- Biallelic loxP insertion: Precise site-specific insertion of loxP sites flanking the target gene in diploid ESCs.
- Simultaneous integration: Tissue-specific Cre drivers (e.g., Alb-Cre, Nes-Cre, Tamoxifen-inducible CreERT2) can be integrated into safe harbor loci or the same chromosome simultaneously
- In vitro co-culture and transfer: Positive ESCs are screened and co-cultured with tetraploid embryos in vitro, developed to the blastocyst stage, and transferred to surrogate mice → surrogate delivery
- Direct genotype generation: Whole-body cells of the pups originate from the edited ESCs → F0 mice are fully genotyped directly, eliminating the need for germline transmission verification
Essential Difference from Traditional “Flox Mouse × Cre Mouse Crossing”: Traditional = Animal-level breeding; TurboMice™ = Cell-level customization + Embryo-level direct production.
Traditon cKO vs TurboMice™ cKO
| Comparison Metrics | Traditional Method (Pronuclear Injection / ES Chimerism) | VeloGene TurboMice™ CKO Customization |
|---|---|---|
| Time to Cre⁺Flox Homozygous Experimental Mice | 12–18 months | 4–6 months |
| Multi-locus Editing on the Same Chromosome (flox + cre + mutation) | Extremely difficult / Genetic drift & loss of control | Feasible via single ESC editing |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Identical clone-level uniformity via same-strain ESCs |
| Direct Usability of F0 for Experiments | No (mostly heterozygous / chimeric) | Yes, F0 mice are directly homozygous with full genotypes |
| Live Birth Efficiency | High fluctuation in chimera rates | 30%–60% post-VeloGene optimization |
| Cryopreservation & Recovery | Sperm cryopreservation, recovery takes 1 year | ESC cryopreservation; batch delivery of Cre⁺Flox homozygotes within 2 months post-recovery |
VeloGene Biotechnology's Conditional Knockout Mouse Solutions Advantages
VeloGene Biotech's TurboMice™ cKO Service Can Cover Complex Scenarios
- Single-gene conditional knockout (Cre⁺ / Flox⁻/⁻)
- Single-Chromosome Multiplex Editing for Co-delivery of Floxed Sites, Cre Cassette, and Reporter Gene
- Dual-gene / triple-gene conditional knockouts (multi-loxP arrays)
- Large-fragment humanization (>20 kb) + flox combination · Inducible CKO (CreERT2) + site-specific point mutations
- Specific strain backgrounds: C57BL/6, BALB/c, etc.
VeloGene's Delivery Period(As quick as in 4 months)
- Editing Strategy Customization: 1–2 days
- Vector Construction: 4–6 weeks
- ESC Editing + Monoclonal Screening & Validation: 4–8 weeks
- Tetraploid Complementation Mouse Production: 4–8 weeks
- Mouse Weaning, Quality Control & Shipping: Approx. 4 weeks
VeloGene Biotechnology's Specialties
- Zero Genetic Drift: Same-batch homologous ESC clones ensure extremely low individual variability
- Ready-to-Use F0: No longer hindered by chimera rates or germline transmission loss
- Reproducible Complex Genotypes: Multi-locus combinations on the same chromosome no longer rely on “luck-based breeding”
- Ultra-Fast Batch Delivery: Initial CKO founder mice delivered in 4–6 months, with subsequent cohort expansion delivered in batches within 2 months
Cre⁺/Flox Homozygous CKO Mice Delivered in as Fast as 4–6 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
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Frequent Asks
Conditional knockout mice traditionally take a year. Can you really deliver in 4 months?
“Traditional breeding involves generating Flox mice + introducing Cre mice + double-heterozygous mating + homozygous screening; TurboMice™ simultaneously integrates flox and cre elements at the ESC stage and directly produces F0 homozygotes via tetraploid complementation, enabling experimental-ready mice delivery in 4–6 months.”
Can two loci on the same chromosome be floxed together?
Yes. Multi-round editing at the ESC level is not limited by meiotic linkage, and VeloGene has delivered dual/triple-target and over-100-kb fragment editing orders.
Can F0-generation CKO mice be directly used for tissue-specific phenotypic assays?
Yes. F0 cells uniformly carry Cre⁺ and flox⁻/⁻ across the entire body, allowing tissue-specific recombination to be triggered via inducers or endogenous promoters without further breeding.
How are subsequent mouse orders handled?
Cryopreserved ESCs are thawed for a batch delivery in 2 months, maintaining a consistent genetic background.