The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
VeloGene Biotechnology
Mouse Customization - Transgenic Service
Beyond Random Insertion | F0 Ready - to -Use
VeloGene Biotechnology Transgenic Mouse Model Customizing Service
Beyond Random Integration: TurboMice™ Clone-Level Uniform Models Delivered in 3–5 Months
Tradition Transgenic vs TurboMice™ Transgenic
| Metrics | Traditional Pronuclear Injection Transgenic Mice | VeloGene TurboMice™ Transgenic Customization |
|---|---|---|
| Gene Integration Method | Random integration, multi-copy anomalies common | ESC site-specific integration, monoclonal origin |
| Time to Obtain Usable Experimental Mice | 6–12 months (requires founder screening + multi-generation breeding) | Delivery in 3–5 months |
| Expression Consistency | Large individual variations, prone to expression silencing | F0 is homozygous, uniform and stable expression |
| Complex Structure Construction | Extremely difficult to achieve large fragments or complex arrangements | Supports >20 kb long fragments and multi-component editing |
| Cryopreservation & Expansion | Relies on sperm/embryo, recovery and breeding takes 1 year+ | ESC cryopreservation, batch delivery within 2 months |
VeloGene Biotechnology's Transgenic Mouse Solutions Advantages
- Site-specific Knock-in (KI): Precisely insert reporter genes (e.g., EGFP, mCherry) and Cre recombinase into specific loci (e.g., Rosa26) to achieve stable and uniform expression.
- Large-Fragment Functional Element Overexpression: Over 20 kb long fragments (e.g., pathogenic genes containing complete regulatory elements, human BAC equivalent replacements), solving the problem of expression instability caused by random integration.
- Multi-Gene Array / Complex Structures: Construct complex transgenic vectors containing multiple promoters, regulatory elements, or dual/triple genes, used to simulate multi-gene regulatory networks or build complex disease models.
- Conditional Transgenic Models: Combined with the Cre-LoxP system, achieving spatiotemporally specific gene expression regulation.
VeloGene's Delivery Period(As quick as in 3 months)
- Strategy Customization & Vector Construction: 1–4 weeks
- ESC Editing & Monoclonal Screening and Validation: 3–6 weeks
- Tetraploid Compensation Mouse Generation: 4–6 weeks
- Weaning, QC, and Shipping: ~3 weeks
Why Choose VeloGene Biotechnology and TurboMice™ for Custom Transgenic Mouse Models?
VeloGene Biotechnology is the pioneer team in the industrialization of TurboMice™ tetraploid complementation technology, raising the live birth rate of tetraploid embryos from the 1–5% reported in literature to 30%–60%, truly replacing ‘mouse breeds mice’ with ‘cell to mice.’ The platform has successfully delivered a large number of complex transgenic models, including large-fragment functional gene overexpression, multi-target humanization, and complex conditional models, providing highly reproducible animal models for drug screening and mechanistic studies.
How to get your mice?
Send Your Inquiry
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Solution Design
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TurboMicing
We will get hands on your project.
Get your Ready to Use f0 Mice!
Our f0 homozygous mice will ship to you and lab-ready!
Frequent Asks
Traditional transgenic mouse founders show large individual variations. How do you ensure consistency?
Traditional methods rely on pronuclear injection, where exogenous genes randomly integrate into the genome, leading to varying copy numbers and integration sites that trigger expression differences. TurboMice™ achieves site-specific integration at the ESC stage, and all F0 mice originate from the same verified ESC monoclonal line, resulting in high genotype and phenotype consistency and completely resolving the issue of genetic drift.
My gene fragment exceeds 20 kb. Can this be done?
Absolutely. ESC homologous recombination is not subject to the capacity limitations of pronuclear injection. MingCeler has successfully delivered multiple long-fragment transgenic projects exceeding 20 kb as well as multi-locus combination projects, ensuring stable integration and expression of large fragments.
Can F0 generation transgenic mice be used directly for efficacy or phenotypic experiments?
Positive. The F0 generation of TurboMice™ consists of homozygotes whose somatic cells uniformly carry the transgene, and their genotypes and other factors undergo strict QC validation, allowing them to proceed directly to efficacy evaluations or basic phenotypic studies in fields such as oncology, immunology, and metabolism.
What if I want more mice in the future?
We cryopreserve the original ESC clones used to build your model. When additional mice are needed, direct thawing and tetraploid compensation production can be performed, enabling batch delivery of live mice in just 2 months with an identical genetic background to the initial batch.
IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.