IL-24 flox Mouse Model
| Starin Name | C57BL/6N‑Il24<sup>tm1(flox)</sup> |
|---|---|
| Strain Background | C57BL/6N |
Applications
- Tumor Immunotherapy & Cancer Biology: Tissue‑ or tumor‑specific IL‑24 ablation to investigate its pro‑apoptotic (ER stress–ROS pathway), anti‑angiogenic (VEGF/MMP‑9 downregulation), and tumor‑microenvironment modulatory functions.
- Immune Inflammation & Metabolic Regulation: Conditional knockout in myeloid, T‑cell, or epithelial compartments to study IL‑24 signaling via IL‑20R1/IL‑20R2 or IL‑22R1/IL‑20R2 and its dual role in Th17 suppression vs. barrier‑disruptive inflammation (e.g., IBD, atopic dermatitis).
- Fibrosis Mechanism Exploration: Organ‑specific (e.g., liver, skin, lung) IL‑24 deletion to assess its contribution to fibrotic progression and myofibroblast activation.
- Tissue Regeneration & Injury Repair: Conditional loss‑of‑function studies in keratinocytes, fibroblasts, or hypoxic tissues to evaluate IL‑24’s HIF‑1α–STAT3 feedback loop in epithelial regeneration and wound closure.
- Cytokine Signaling & STAT Pathway Research: Dissecting JAK/STAT and p38 MAPK axis activation downstream of IL‑24 receptor engagement in a cell‑type–restricted manner.
Key Features
- Classic flox Design: Critical exon(s) of Il24are flanked by two loxP sites; Cre‑mediated recombination efficiently deletes the target exon, producing a null allele in Cre‑expressing tissues.
- Preserved Baseline Phenotype: Homozygous floxed mice express normal IL‑24 levels prior to Cre exposure—viable, fertile, and free of the complex multi‑organ phenotypes associated with constitutive Il24knockout.
- Tissue‑Specific Dissection: Enables organ‑ or cell‑type–restricted knockout (e.g., K14‑Cre for epidermis, LysM‑Cre for macrophages, CD4‑Cre for T cells, or inducible Cre‑ERT2 lines), eliminating off‑target interpretive confounds.
- Genotypically & Transcriptionally Validated: Correct 5′/3′ loxP insertion confirmed by PCR; Cre‑dependent mRNA splicing/excision verified—colony is stable, reproducible, and maintainable as homozygotes.
Strain Description
IL‑24flox/flox(Interleukin‑24 Conditional Knockout) Mouse Model — C57BL/6N‑Il24tm1(flox)/MCL
This model carries loxP sites flanking the critical coding exon(s) of the endogenous murine Il24(melanoma differentiation‑associated gene‑7, Mda‑7) on the C57BL/6N background. Crossing with tissue‑specific Cre deleter strains enables spatiotemporally controlled deletion of IL‑24, permitting dissection of its roles in tumor suppression, immune modulation, wound healing, and fibrotic or inflammatory disease—without the confounding systemic effects of global Il24knockout.
FAQ
Game-changing benefits？
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