hBDCA2 Mouse Model
| Starin Name | C57BL/6N‑Rosa26<sup>tm1(hBDCA2‑pBDCA2)</sup> |
|---|---|
| Strain Background | C57BL/6N |
Applications
- pDC Function & Antiviral Immunity: Investigating TLR7/9–mediated type I IFN production, pDC activation thresholds, and pDC-driven antiviral immune responses in vivo.
- Autoimmune & Inflammatory Disease Modeling: Studying pDC hyperactivation, “type I interferon signature,” and BDCA-2–mediated inhibitory signaling in systemic lupus erythematosus (SLE), psoriasis, and chronic inflammatory contexts.
- Tumor Immunology & Immune Evasion: Dissecting pDC roles in the tumor microenvironment—both as antitumor immune initiators and as inducers of regulatory T cells promoting immune suppression—and testing BDCA-2–targeting agonists/antibodies.
- Immunotherapy Target Validation & Efficacy: Preclinical PK/PD and efficacy assessment of anti-BDCA-2 agonistic/antagonistic antibodies, Fc-engineered formats, or BDCA-2–directed CAR constructs.
- Immune Cell Lineage & Signaling Studies: Examining BDCA-2–FcRγ–ITAM/ITIM cascade and its downstream inhibition of TLR-MyD88–IRF7 signaling in pDCs.
Key Features
- pDC-Restricted Human BDCA-2 Expression: Human BDCA-2 is expressed under its native promoter exclusively in pDCs (splenic & BM pDCs positive; T/B/NK cells negative), faithfully recapitulating the human pDC surface marker profile.
- Rosa26Safe Harbor Integration: Inserted at the Rosa26locus ensuring consistent single-copy expression, homogeneous copy number, stable inheritance, and minimal positional-effect variegation.
- Preserved Immune Homeostasis: Flow cytometric validation confirms unaffected proportions of T cells, B cells, NK cells, and pDCs—immune subset composition indistinguishable from WT C57BL/6N mice.
- Functional Inhibitory Signaling Capability: Human BDCA-2 on pDCs can be cross-linked to deliver inhibitory signals via associated FcRγ chain, suppressing TLR7/9–dependent IFN-α/cytokine production—enabling functional interrogation of BDCA-2–mediated immune modulation.
Strain Description
hBDCA2 (hCLEC4C) Humanized Knock-in Mouse Model — C57BL/6N‑Rosa26tm1(hBDCA2‑pBDCA2)/MCL
This model harbors a targeted knock-in of the human BDCA2(CLEC4C/CD303) coding sequence together with its native promoter inserted into the conserved Rosa26safe harbor locus on the C57BL/6N background. Human BDCA-2 is selectively expressed on plasmacytoid dendritic cells (pDCs) with physiological pDC development and immune homeostasis preserved, providing a translatable in vivoplatform for pDC functional studies, autoimmune/inflammatory disease modeling, and evaluation of BDCA-2–targeted immunotherapies.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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