Arg2- flox Mouse Model
| Starin Name | C57BL/6N‑Arg2<sup>tm1(flox)</sup> |
|---|---|
| Strain Background | C57BL/6N |
Applications
- Immune Metabolism & Macrophage Polarization: Conditional deletion in myeloid cells to study Arg2‑mediated metabolic reprogramming (M2‑like anti‑inflammatory phenotype, IL‑10 response) in macrophages.
- T‑cell Immunometabolism & Tumor Immunity: T‑cell–specific Arg2 ablation (e.g., CD4‑Cre, CD8‑Cre) to evaluate its role as a cell‑autonomous regulator of CD8⁺ cytotoxic T‑cell function and antitumor efficacy.
- Cancer Metabolism Research: Tumor‑ or stroma‑specific Arg2 knockout to investigate mitochondrial Arg2 effects on ROS generation (mtROS–Sirt3 axis), proliferation, migration, and chemoresistance in solid tumors.
- Liver / Kidney Fibrosis & Amino Acid Homeostasis: Hepatocyte‑ or renal tubular‑specific deletion to explore Arg2 involvement in arginine metabolism, urea‑cycle branch activity, fibrosis progression, and NO‑pathway modulation.
- Target Validation for Immuno‑metabolic Therapies: Preclinical assessment of Arg2‑targeted intervention strategies in metabolic and oncologic contexts.
Key Features
- Classic flox Design: Critical exon(s) of Arg2are flanked by two loxP sites; Cre‑mediated recombination results in efficient frameshift deletion in Cre‑expressing tissues.
- Preserved Baseline: Heterozygous and homozygous floxed mice show normal Arg2 expression and physiological phenotypes prior to Cre recombination—no embryonic lethality or baseline metabolic defect.
- Tissue‑Specific Dissection: Enables organ‑ or cell‑type–restricted knockout (e.g., LysM‑Cre for macrophages, Alb‑Cre for hepatocytes, CD4/CD8‑Cre for T cells), eliminating interpretive confounds from systemic Arg2loss.
- Genotypically Validated: Correct 5′‑ and 3′‑loxP insertion confirmed by PCR; Cre‑dependent mRNA excision verified—model is stable, reproducible, and colony‑maintainable as homozygotes.
Strain Description
Arg2flox/flox(Arginase‑2 Conditional Knockout) Mouse Model — C57BL/6N‑Arg2tm1(flox)/MCL
This model carries loxP sites flanking exon(s) of the murine Arg2(arginase‑2) gene on the C57BL/6N background. Crossing with tissue‑specific Cre deleter strains enables spatially and temporally controlled deletion of Arg2, allowing dissection of mitochondrial arginase‑2 function in amino acid metabolism, immune cell polarization, and tumor‑microenvironment biology without the confounding phenotypes of global Arg2knockout.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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