The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
VeloGene Biotechnology
Knockin (KI) Mouse
for Research
From Cell to Mice by TurboMice™
- AAALAC
- ISO
- F0 Direct Usability: Homozygous mice ready for experiments instantly.
- 3–5 Month Delivery: 2× faster than the traditional 6–12 month timeline.
- Minimum Genetic Drift: Clone-level uniformity with absolute background consistency.
- IP Ownership: Strict fee-for-service model with absolute protection and no third-party resale.
- 2-Year Free ESC Banking: Effortless batch re-ordering in just 2 months.
- SPF Level Delivery From Door to Door
Get Your Project Evaluated by Our Scientist for Free
Or contact us here!
* Get a free, no-obligation feasibility assessment for your mouse project.
* All project information shared is strictly confidential, and your IP rights are fully protected.
Our team will respond within 24 working hours. We will not make unsolicited phone calls.
How Long it Takes to Get My Lab-Ready F0 Knockin Mice?
The next generation of mouse model preparation platform. By using tetraploid complementation technology, TurboMice™ allows us to shorten the time to 3-5 months. 2× faster than the traditional 6–12 month workflow.
4 - 6 Weeks→
Designing & Targeting Vector Constructing
4 - 6 Weeks→
Cell Editing & Screening
3 - 4 Weeks→
Tetraploid Mouse Producing
3 Weeks→
Weaned & Ready to Transport
Total:
3-5 Months
SPF level stable delivery from door to door
Re-ordering Mice in the Same Gene in 45 Days
by TurboMice™ ES Banking Technology
Leveraging advanced sperm cryopreservation, purification, and rederivation, we help you bypass traditional breeding bottlenecks. Whether dealing with limited male mice, pathogen contamination risks, or mating failures, our technology allows you to repeatedly obtain genetically identical, pathogen-free mouse cohorts with uniform ages in just about 45 days—saving time, cost, and animal housing space while securing your valuable genetic resources.
How to Get my
Knockin (KI)
Mouse Model?
We will determine the best approach for your desired knockin mouse model base on your requirements.
Send Your Inquiry
Before we start, you can start by submitting your inquiry form or talk to us on our Whatsapp and tell us what you need!
Solution Design
We will reply to you as soon as possible and send you a quote!
TurboMicing
We will get hands on your project.
Get your Ready to Use f0 Mice!
Our f0 homozygous mice will ship to you and lab-ready!
Full Qualification
VeloGene Biotechnology Shares the Qualifications of Its Parent Company - MingCeler Biotech
Including AAALAC, ISO, and China Laboratory Animal Production License
Clients Across the Continents
Our Clients Are Located Throughout the Asia Pacific, North America, and Europe with Seamless Global Delivery
Backed by our robust global delivery capabilities extending to clients in South Korea, the US, Germany, Sweden, and beyond, our reliable logistics and seamless customs compliance serve as your strongest guarantee, ensuring a stable, secure door-to-door journey from our facility straight to your lab using custom-built transport cages that fully comply with animal welfare standards, strict SPF-grade requirements, and multi-country import-export quarantine regulations for smooth customs clearance worldwide.
Client Cases
Scroll Down to See Technical Details on VeloGene Biotechnology's
Knockin Mouse Model Technical Details
Comparison, Advantages and FAQ
VeloGene Biotechnology Knockin Mouse Model Customizing Service
F0 "Founder" Ready - to -Use
Knock-in (KI) mice refer to genetically modified models in which exogenous sequences (such as reporter genes, tag sequences, mutation sites, or humanized fragments) are precisely inserted into specific genomic sites (typically endogenous gene sites) using gene-editing technology, enabling site-specific modification and functional studies of the target gene.
VeloGene’s TurboMice™ tetraploid complementation technology completes in situ KI editing at the embryonic stem cell (ESC) level, followed by aggregation with tetraploid embryos (which form only extraembryonic tissues such as the placenta) to compensate development.
In Situ ESC-Level Editing: Completes precise gene-editing at the embryonic stem cell level for high genetic fidelity.
Direct F0 Generation Homozygosity: All F0 generation mice originate from the same edited ESC monoclonal line, delivering homozygous and stably heritable KI models directly.
Rapid Delivery (3–5 Months): Achieves full generation and delivery within 3 to 5 months.
No Multi-Generation Breeding: Completely skips traditional multi-generation backcrossing and screening workflows.
Tradition Knockin vs TurboMice™ Knockin
| Metrics | Traditional ES Chimerism / Pronuclear Injection / CRISPR | VeloGene TurboMice™ KI Customization |
|---|---|---|
| Time to Obtain Homozygous Usable KI Mice | 6–12 months (multi-generation intercrossing & screening) | Delivery in 3–5 months |
| Long-Fragment / Multi-Component Knock-in (>10 kb) | Low efficiency, prone to random insertion, unstable expression | ESC homologous recombination, *in situ* precise integration |
| Same-Chromosome Multi-locus KI Combinations | Linkage drift, out-of-control breeding cycles | One-time multi-locus knock-in via ESC monoclonal lines |
| F0 Direct Experimental Usability | Mostly chimeric / heterozygous, requires expansion validation | Yes, F0 mice are directly homozygous with full genotypes |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Clone-level uniformity via same-strain ESCs |
| Cryopreservation & Expansion | Sperm/embryo cryopreservation, recovery & breeding takes 1 year+ | ESC cryopreservation, batch delivery within 2 months |
VeloGene Biotechnology's Knockin Mouse Solutions Advantages
VeloGene Biotech's TurboMice™ Knockin Service Can Cover Complex Scenarios
- Reporter Gene Knock-in (e.g., Rosa26 or Col1a1 locus knock-in of EGFP/Luciferase/T2A-Cre)
- Tag Protein Knock-in (e.g., 3×FLAG/HA/Myc fusion expression for protein interaction and localization studies)
- Human Sequence / Functional Domain Knock-in (e.g., humanized antibody constant regions, immune checkpoint extracellular domain replacement)
- Conditional Knockout (Flox) Mouse Generation (precise introduction of LoxP sites to prevent embryonic lethality)
- Dual/Triple-Gene KI (e.g., multi-reporter gene co-expression, multi-target gene editing screening models)
VeloGene's Delivery Period(As quick as in 3 months)
- Editing Strategy Customization: 1–2 days
- Vector Construction: 2–4 weeks
- ESC Editing & Monoclonal Validation (PCR/Sanger/WB): 3–6 weeks
- Tetraploid Compensation Mouse Generation: 4–6 weeks
- Weaning, QC, and Shipping: ~3 weeks
Homozygous Knockin Mice Delivered in as Fast as 3-5 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
Frequent Asks
Knockin mice from scratch traditionally take more than 6 months. Can you really deliver in 3 months?
Traditional method involves vector construction + ES chimerism + germline transmission + homozygous screening; whereas TurboMice™ completes the humanization all at once at the ESC level, with founder mice (F0) being directly homozygous, enabling the delivery of experiment-ready mice within 3 to 5 months.”
Is >50kb long fragment humanizing really possible?
Affirmative. ESC homologous recombination is not limited by the DNA capacity constraints of pronuclear injection; VeloGene has delivered long-fragment KI orders exceeding 50 kb as well as multi-locus combination projects, ensuring site-specific integration and stable expression of exogenous sequences.
Can F0 generation genetically knockin mice be used directly for pharmacodynamic experiments?
Positive. All somatic cells in F0 mice uniformly carry the homozygous KI genotype, and the expression of reporter genes/tag proteins is strictly validated by fluorescence detection/WB QC, allowing them to proceed directly to experiments such as cell tracing, protein interaction, and gene therapy evaluation without further expansion breeding.
What if I want more mice in the future?
Cryopreserved ESC clones are directly revived to edit lines, delivering live animals in batches within 2 months, featuring identical strain and origin with zero genetic drift.
Related Products
How to get your mice?
Send Your Inquiry
Before we start, you can start by submitting your inquiry form or talk to us on our Whatsapp and tell us what you need!
Solution Design
We will reply to you as soon as possible and send you a quote!
TurboMicing
We will get hands on your project.
Get your Ready to Use f0 Mice!
Our f0 homozygous mice will ship to you and lab-ready!
IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.