Aldh1l1-P2A-iCre Mouse Model
| Starin Name | C57BL/6N‑Aldh1l1<sup>tm1(P2A‑iCre)</sup> |
|---|---|
| Strain Background | C57BL/6N |
Applications
- Astrocyte Biology & Lineage Tracing: Crossing with Cre‑dependent reporter strains (e.g., mT/mG, Rosa26tdTomato) to trace astrocyte development, heterogeneity, and fate mapping across brain regions.
- Neurodegenerative Disease Research: Astrocyte‑specific conditional knockout in models of Alzheimer’s disease, Parkinson’s disease, ALS, and aging‑related neurodegeneration.
- CNS Injury & Repair: Studying astrocytic roles in traumatic brain injury, spinal cord injury, neuroinflammation (encephalitis, multiple sclerosis), and blood‑brain barrier maintenance.
- Liver & Kidney Metabolic Disease: Hepatocyte‑ or renal proximal tubule‑specific gene manipulation to investigate NAFLD/NASH, drug‑induced hepatotoxicity, and renal injury‑repair mechanisms.
- Glia‑Neuron Interaction & Neural Circuit Homeostasis: Dissecting astrocyte‑to‑neuron signaling pathways and astrocytic contribution to synaptic modulation and neural micro‑environment stability.
Key Features
- P2A Self‑Cleavage Ensures Dual Functionality: P2A peptide mediates >90% efficient co‑translation and self‑cleavage, yielding independent, fully active Aldh1l1 and iCre proteins without fusion‑protein artifacts.
- Faithful Lineage‑Specific Cre Expression: iCre mirrors the endogenous Aldh1l1 spatiotemporal expression pattern—highly specific to astrocytes (≥91.7% targeting even in GFAP‑low regions such as thalamus/cerebellum), hepatocytes, and renal proximal tubules—with negligible leak or off‑target recombination.
- Preserved Endogenous Gene Function: Aldh1l1ORF and regulatory elements remain intact; homozygous mice are viable, fertile, and metabolically/neurologically normal.
- Superior to GFAP‑Cre Lines: Overcomes regional blind spots of GFAP‑driven Cre tools, enabling comprehensive CNS astrocyte targeting across all major brain regions.
Strain Description
Aldh1l1‑P2A‑iCre Knock‑in Mouse Model (C57BL/6N‑Aldh1l1tm1(P2A‑iCre)/MCL)
This model is generated by precise insertion of a P2A‑iCre (improved Cre recombinase) cassette immediately after the stop codon of the endogenous Aldh1l1gene on the C57BL/6N background. Aldh1l1 and iCre are co‑translated as a single polypeptide and cleaved by the P2A peptide in vivo, ensuring faithful, lineage‑specific Cre expression in Aldh1l1‑positive cells (central nervous system astrocytes, hepatocytes, renal proximal tubule epithelial cells) while preserving native Aldh1l1 protein function. It enables Cre‑mediated conditional gene recombination exclusively within the Aldh1l1 lineage.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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