The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
VeloGene Biotechnology
Mouse Customization - Knockin Service
F0 Ready - to -Use
VeloGene Biotechnology Knockin Mouse Model Customizing Service
F0 "Founder" Ready - to -Use
Knock-in (KI) mice refer to genetically modified models in which exogenous sequences (such as reporter genes, tag sequences, mutation sites, or humanized fragments) are precisely inserted into specific genomic sites (typically endogenous gene sites) using gene-editing technology, enabling site-specific modification and functional studies of the target gene.
VeloGene’s TurboMice™ tetraploid complementation technology completes in situ KI editing at the embryonic stem cell (ESC) level, followed by aggregation with tetraploid embryos (which form only extraembryonic tissues such as the placenta) to compensate development.
In Situ ESC-Level Editing: Completes precise gene-editing at the embryonic stem cell level for high genetic fidelity.
Direct F0 Generation Homozygosity: All F0 generation mice originate from the same edited ESC monoclonal line, delivering homozygous and stably heritable KI models directly.
Rapid Delivery (3–5 Months): Achieves full generation and delivery within 3 to 5 months.
No Multi-Generation Breeding: Completely skips traditional multi-generation backcrossing and screening workflows.
Tradition Knockin vs TurboMice™ Knockin
| Metrics | Traditional ES Chimerism / Pronuclear Injection / CRISPR | VeloGene TurboMice™ KI Customization |
|---|---|---|
| Time to Obtain Homozygous Usable KI Mice | 6–12 months (multi-generation intercrossing & screening) | Delivery in 3–5 months |
| Long-Fragment / Multi-Component Knock-in (>10 kb) | Low efficiency, prone to random insertion, unstable expression | ESC homologous recombination, *in situ* precise integration |
| Same-Chromosome Multi-locus KI Combinations | Linkage drift, out-of-control breeding cycles | One-time multi-locus knock-in via ESC monoclonal lines |
| F0 Direct Experimental Usability | Mostly chimeric / heterozygous, requires expansion validation | Yes, F0 mice are directly homozygous with full genotypes |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Clone-level uniformity via same-strain ESCs |
| Cryopreservation & Expansion | Sperm/embryo cryopreservation, recovery & breeding takes 1 year+ | ESC cryopreservation, batch delivery within 2 months |
VeloGene Biotechnology's Knockin Mouse Solutions Advantages
VeloGene Biotech's TurboMice™ Knockin Service Can Cover Complex Scenarios
- Reporter Gene Knock-in (e.g., Rosa26 or Col1a1 locus knock-in of EGFP/Luciferase/T2A-Cre)
- Tag Protein Knock-in (e.g., 3×FLAG/HA/Myc fusion expression for protein interaction and localization studies)
- Human Sequence / Functional Domain Knock-in (e.g., humanized antibody constant regions, immune checkpoint extracellular domain replacement)
- Conditional Knockout (Flox) Mouse Generation (precise introduction of LoxP sites to prevent embryonic lethality)
- Dual/Triple-Gene KI (e.g., multi-reporter gene co-expression, multi-target gene editing screening models)
VeloGene's Delivery Period(As quick as in 3 months)
- Editing Strategy Customization: 1–2 days
- Vector Construction: 2–4 weeks
- ESC Editing & Monoclonal Validation (PCR/Sanger/WB): 3–6 weeks
- Tetraploid Compensation Mouse Generation: 4–6 weeks
- Weaning, QC, and Shipping: ~3 weeks
Homozygous Knockin Mice Delivered in as Fast as 3-5 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
Frequent Asks
Knockin mice from scratch traditionally take more than 6 months. Can you really deliver in 3 months?
Traditional method involves vector construction + ES chimerism + germline transmission + homozygous screening; whereas TurboMice™ completes the humanization all at once at the ESC level, with founder mice (F0) being directly homozygous, enabling the delivery of experiment-ready mice within 3 to 5 months.”
Is >50kb long fragment humanizing really possible?
Affirmative. ESC homologous recombination is not limited by the DNA capacity constraints of pronuclear injection; VeloGene has delivered long-fragment KI orders exceeding 50 kb as well as multi-locus combination projects, ensuring site-specific integration and stable expression of exogenous sequences.
Can F0 generation genetically knockin mice be used directly for pharmacodynamic experiments?
Positive. All somatic cells in F0 mice uniformly carry the homozygous KI genotype, and the expression of reporter genes/tag proteins is strictly validated by fluorescence detection/WB QC, allowing them to proceed directly to experiments such as cell tracing, protein interaction, and gene therapy evaluation without further expansion breeding.
What if I want more mice in the future?
Cryopreserved ESC clones are directly revived to edit lines, delivering live animals in batches within 2 months, featuring identical strain and origin with zero genetic drift.
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IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.