The Next Generation of Mouse Model Preparation Platform
VeloGene Biotechnology
Mouse Customize - Conditional Knockout(cKO) service
F0 Cre⁺fl/fl Ready - to -Use
VeloGene Biotechnology Condition Knockout(cKO) Mouse Model Customize Service
F0 "Founder" Cre⁺fl/fl Ready - to -Use
What are "Conditional Knockout Mice" under the TurboMice™ Tetraploid Complementation Route?
- Biallelic loxP insertion: Precise site-specific insertion of loxP sites flanking the target gene in diploid ESCs.
- Simultaneous integration: Tissue-specific Cre drivers (e.g., Alb-Cre, Nes-Cre, Tamoxifen-inducible CreERT2) can be integrated into safe harbor loci or the same chromosome simultaneously
- In vitro co-culture and transfer: Positive ESCs are screened and co-cultured with tetraploid embryos in vitro, developed to the blastocyst stage, and transferred to surrogate mice → surrogate delivery
- Direct genotype generation: Whole-body cells of the pups originate from the edited ESCs → F0 mice are fully genotyped directly, eliminating the need for germline transmission verification
Essential Difference from Traditional “Flox Mouse × Cre Mouse Crossing”: Traditional = Animal-level breeding; TurboMice™ = Cell-level customization + Embryo-level direct production.
Traditon cKO vs TurboMice™ cKO
| Comparison Metrics | Traditional Method (Pronuclear Injection / ES Chimerism) | VeloGene TurboMice™ CKO Customization |
|---|---|---|
| Time to Cre⁺Flox Homozygous Experimental Mice | 12–18 months | 4–6 months |
| Multi-locus Editing on the Same Chromosome (flox + cre + mutation) | Extremely difficult / Genetic drift & loss of control | Feasible via single ESC editing |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Identical clone-level uniformity via same-strain ESCs |
| Direct Usability of F0 for Experiments | No (mostly heterozygous / chimeric) | Yes, F0 mice are directly homozygous with full genotypes |
| Live Birth Efficiency | High fluctuation in chimera rates | 30%–60% post-VeloGene optimization |
| Cryopreservation & Recovery | Sperm cryopreservation, recovery takes 1 year | ESC cryopreservation; batch delivery of Cre⁺Flox homozygotes within 2 months post-recovery |
VeloGene Biotechnology's Conditional Knockout Moue Solutions Advantages
VeloGene Biotech's TurboMice™ cKO Service Can Cover Complex Scenarios
- Single-gene conditional knockout (Cre⁺ / Flox⁻/⁻)
- Single-Chromosome Multiplex Editing for Co-delivery of Floxed Sites, Cre Cassette, and Reporter Gene
- Dual-gene / triple-gene conditional knockouts (multi-loxP arrays)
- Large-fragment humanization (>20 kb) + flox combination · Inducible CKO (CreERT2) + site-specific point mutations
- Specific strain backgrounds: C57BL/6, BALB/c, etc.
VeloGene's Delivery Period(As quick as in 4 months)
- Editing Strategy Customization: 1–2 days
- Vector Construction: 4–6 weeks
- ESC Editing + Monoclonal Screening & Validation: 4–8 weeks
- Tetraploid Complementation Mouse Production: 4–8 weeks
- Mouse Weaning, Quality Control & Shipping: Approx. 4 weeks
VeloGene Biotechnology's Specialties
- Zero Genetic Drift: Same-batch homologous ESC clones ensure extremely low individual variability
- Ready-to-Use F0: No longer hindered by chimera rates or germline transmission loss
- Reproducible Complex Genotypes: Multi-locus combinations on the same chromosome no longer rely on “luck-based breeding”
- Ultra-Fast Batch Delivery: Initial CKO founder mice delivered in 4–6 months, with subsequent cohort expansion delivered in batches within 2 months
Cre⁺/Flox Homozygous CKO Mice Delivered in as Fast as 4–6 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
Related Products
Frequent Asks
Conditional knockout mice traditionally take a year. Can you really deliver in 4 months?
“Traditional breeding involves generating Flox mice + introducing Cre mice + double-heterozygous mating + homozygous screening; TurboMice™ simultaneously integrates flox and cre elements at the ESC stage and directly produces F0 homozygotes via tetraploid complementation, enabling experimental-ready mice delivery in 4–6 months.”
Can two loci on the same chromosome be floxed together?
Yes. Multi-round editing at the ESC level is not limited by meiotic linkage, and VeloGene has delivered dual/triple-target and over-100-kb fragment editing orders.
Can F0-generation CKO mice be directly used for tissue-specific phenotypic assays?
Yes. F0 cells uniformly carry Cre⁺ and flox⁻/⁻ across the entire body, allowing tissue-specific recombination to be triggered via inducers or endogenous promoters without further breeding.
How are subsequent mouse orders handled?
Cryopreserved ESCs are thawed for a batch delivery in 2 months, maintaining a consistent genetic background.
IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.