The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
VeloGene Biotechnology
Conditional Knockout (cKO) Mouse for Research
From Cell to Mice by TurboMice™
- AAALAC
- ISO
- F0 Direct Usability: Genefl/fl, Cre+ ready for experiments instantly.
- 4–6 Month Delivery: 2× faster than the traditional 12–18 month timeline.
- Complex Multi-Site Customization: Simultaneous integration of flox, Cre, and mutations on the same chromosome.
- Minimum Genetic Drift: Clone-level uniformity with absolute background consistency.
- IP Ownership: Strict fee-for-service model with absolute protection and no third-party resale.
- 2-Year Free ESC Banking: Effortless batch re-ordering in just 2 months.
- SPF Level Delivery From Door to Door
Get Your Project Evaluated by Our Scientist for Free
Or contact us here!
* Get a free, no-obligation feasibility assessment for your mouse project.
* All project information shared is strictly confidential, and your IP rights are fully protected.
Our team will respond within 24 working hours. We will not make unsolicited phone calls.
How Long it Takes to Get My Lab-Ready F0 Conditional Knockout Mice?
The next generation of mouse model preparation platform. By using tetraploid complementation technology, TurboMice™ allows us to shorten the time to 4 -6 months. 2× faster than the traditional 6–12 month workflow.
6 - 8 Weeks→
Designing & Targeting Vector Constructing
6 - 8 Weeks→
Cell Editing & Screening
3 - 4 Weeks→
Tetraploid Mouse Producing
3 Weeks→
Weaned & Ready to Transport
Total:
4 -6 Months
SPF level stable delivery from door to door
Re-ordering Mice in the Same Gene in 45 Days
by TurboMice™ ES Banking Technology
Leveraging advanced sperm cryopreservation, purification, and rederivation, we help you bypass traditional breeding bottlenecks. Whether dealing with limited male mice, pathogen contamination risks, or mating failures, our technology allows you to repeatedly obtain genetically identical, pathogen-free mouse cohorts with uniform ages in just about 45 days—saving time, cost, and animal housing space while securing your valuable genetic resources.
How to Get my
Conditional Konckout(cKO)
Mouse Model?
We will determine the best approach for your desired cKO mouse model base on your requirements.
Send Your Inquiry
Before we start, you can start by submitting your inquiry form or talk to us on our Whatsapp and tell us what you need!
Solution Design
We will reply to you as soon as possible and send you a quote!
TurboMicing
We will get hands on your project.
Get your Ready to Use f0 Mice!
Our f0 homozygous mice will ship to you and lab-ready!
Full Qualification
VeloGene Biotechnology Shares the Qualifications of Its Parent Company - MingCeler Biotech
Including AAALAC, ISO, and China Laboratory Animal Production License
Clients Across the Continents
Our Clients Are Located Throughout the Asia Pacific, North America, and Europe with Seamless Global Delivery
Backed by our robust global delivery capabilities extending to clients in South Korea, the US, Germany, Sweden, and beyond, our reliable logistics and seamless customs compliance serve as your strongest guarantee, ensuring a stable, secure door-to-door journey from our facility straight to your lab using custom-built transport cages that fully comply with animal welfare standards, strict SPF-grade requirements, and multi-country import-export quarantine regulations for smooth customs clearance worldwide.
Client Cases
Scroll Down to See Technical Details on VeloGene Biotechnology's
cKO Mouse Model Technical Details
Comparison, Advantages and FAQ
VeloGene Biotechnology Condition Knockout(cKO) Mouse Model Customizing Service
F0 "Founder" Cre⁺fl/fl Ready - to -Use
In neuroscience, immunology, metabolism, and oncology research, conditional knockout (CKO) mice are essential tools for dissecting tissue-specific gene functions. Traditional workflows require separately generating Flox mice and Cre driver mice, followed by multiple generations of breeding—taking over a year—while multi-locus editing on the same chromosome is nearly impossible.
VeloGene Biotechnology’s TurboMice™ tetraploid complementation technology completes the introduction of loxP sites + integration of Cre elements at the embryonic stem cell (ESC) stage in a single step. Through tetraploid embryo complementation, 100% of the F0 generation directly yields Cre-positive + Flox homozygous mice(Genefl/fl, Cre+)derived entirely from edited stem cells—bypassing chimeras, skipping breeding generations, and eliminating guesswork-style mating.
What are "Conditional Knockout Mice" under the TurboMice™ Tetraploid Complementation Route?
- Biallelic loxP insertion: Precise site-specific insertion of loxP sites flanking the target gene in diploid ESCs.
- Simultaneous integration: Tissue-specific Cre drivers (e.g., Alb-Cre, Nes-Cre, Tamoxifen-inducible CreERT2) can be integrated into safe harbor loci or the same chromosome simultaneously
- In vitro co-culture and transfer: Positive ESCs are screened and co-cultured with tetraploid embryos in vitro, developed to the blastocyst stage, and transferred to surrogate mice → surrogate delivery
- Direct genotype generation: Whole-body cells of the pups originate from the edited ESCs → F0 mice are fully genotyped directly, eliminating the need for germline transmission verification
Essential Difference from Traditional “Flox Mouse × Cre Mouse Crossing”: Traditional = Animal-level breeding; TurboMice™ = Cell-level customization + Embryo-level direct production.
Traditon cKO vs TurboMice™ cKO
| Comparison Metrics | Traditional Method (Pronuclear Injection / ES Chimerism) | VeloGene TurboMice™ CKO Customization |
|---|---|---|
| Time to Cre⁺Flox Homozygous Experimental Mice | 12–18 months | 4–6 months |
| Multi-locus Editing on the Same Chromosome (flox + cre + mutation) | Extremely difficult / Genetic drift & loss of control | Feasible via single ESC editing |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Identical clone-level uniformity via same-strain ESCs |
| Direct Usability of F0 for Experiments | No (mostly heterozygous / chimeric) | Yes, F0 mice are directly homozygous with full genotypes |
| Live Birth Efficiency | High fluctuation in chimera rates | 30%–60% post-VeloGene optimization |
| Cryopreservation & Recovery | Sperm cryopreservation, recovery takes 1 year | ESC cryopreservation; batch delivery of Cre⁺Flox homozygotes within 2 months post-recovery |
VeloGene Biotechnology's Conditional Knockout Mouse Solutions Advantages
VeloGene Biotech's TurboMice™ cKO Service Can Cover Complex Scenarios
- Single-gene conditional knockout (Cre⁺ / Flox⁻/⁻)
- Single-Chromosome Multiplex Editing for Co-delivery of Floxed Sites, Cre Cassette, and Reporter Gene
- Dual-gene / triple-gene conditional knockouts (multi-loxP arrays)
- Large-fragment humanization (>20 kb) + flox combination · Inducible CKO (CreERT2) + site-specific point mutations
- Specific strain backgrounds: C57BL/6, BALB/c, etc.
VeloGene's Delivery Period(As quick as in 4 months)
- Editing Strategy Customization: 1–2 days
- Vector Construction: 4–6 weeks
- ESC Editing + Monoclonal Screening & Validation: 4–8 weeks
- Tetraploid Complementation Mouse Production: 4–8 weeks
- Mouse Weaning, Quality Control & Shipping: Approx. 4 weeks
VeloGene Biotechnology's Specialties
- Zero Genetic Drift: Same-batch homologous ESC clones ensure extremely low individual variability
- Ready-to-Use F0: No longer hindered by chimera rates or germline transmission loss
- Reproducible Complex Genotypes: Multi-locus combinations on the same chromosome no longer rely on “luck-based breeding”
- Ultra-Fast Batch Delivery: Initial CKO founder mice delivered in 4–6 months, with subsequent cohort expansion delivered in batches within 2 months
Cre⁺/Flox Homozygous CKO Mice Delivered in as Fast as 4–6 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
Related Products
Frequent Asks
Conditional knockout mice traditionally take a year. Can you really deliver in 4 months?
“Traditional breeding involves generating Flox mice + introducing Cre mice + double-heterozygous mating + homozygous screening; TurboMice™ simultaneously integrates flox and cre elements at the ESC stage and directly produces F0 homozygotes via tetraploid complementation, enabling experimental-ready mice delivery in 4–6 months.”
Can two loci on the same chromosome be floxed together?
Yes. Multi-round editing at the ESC level is not limited by meiotic linkage, and VeloGene has delivered dual/triple-target and over-100-kb fragment editing orders.
Can F0-generation CKO mice be directly used for tissue-specific phenotypic assays?
Yes. F0 cells uniformly carry Cre⁺ and flox⁻/⁻ across the entire body, allowing tissue-specific recombination to be triggered via inducers or endogenous promoters without further breeding.
How are subsequent mouse orders handled?
Cryopreserved ESCs are thawed for a batch delivery in 2 months, maintaining a consistent genetic background.
IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.