The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
Why Choose VeloGene Biotechnology?
What Is a Humanized Mouse Model via Tetraploid Complementation?
VeloGene Biotechnology Genetically Humanized Mouse Model Customization Service
Accelerate Preclinical Drug R&D with Experiment-Ready, Homozygous F0 Founders in 3-5 Months
- Core Technology: TurboMice™ tetraploid complementation technology performs in situ humanized editing at the embryonic stem cell (ESC) level.
- Developmental Mechanism: Edited ESCs are aggregated with tetraploid embryos—which exclusively develop into extraembryonic tissues like the placenta—to provide full developmental compensation.
- F0 Generation: All resulting F0 generation founder mice originate from the exact same edited ESC single clone, ensuring 100% genotype uniformity.
- Efficiency & Timeline: Delivers homozygous, stably inheritable humanized mice directly ready for in vivo pharmacodynamic (PD) experiments within a timeframe of 3 to 5 months.
Tradition Humanized vs TurboMice™ Humanized
| Comparison Metrics | Traditional Method (ES Chimerism / Pronuclear Injection / BAC) | VeloGene TurboMice™ Humanized Customization |
|---|---|---|
| Time to Obtain Homozygous Usable Humanized Mice | 6–12 months (multi-generation intercrossing & screening) | 3–5 months for delivery |
| Long-Fragment Humanization (>20 kb) | Extremely difficult; BAC random insertion leads to unstable expression | In situ precise gene editing |
| Multi-locus Editing on the Same Chromosome (Multi-point Humanization) | Linkage drift and out-of-control intercrossing | ESC homologous recombination |
| Direct Usability of F0 for Experiments | Mostly chimeric / heterozygous | Yes, F0 mice are directly homozygous with full genotypes |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Identical clone-level uniformity via same-strain ESCs |
| Cryopreservation & Recovery | Sperm/embryo cryopreservation, recovery & breeding takes 1 year+ | ESC cryopreservation, batch delivery within 2 months post-recovery |
VeloGene Biotechnology's Humanized Mouse Solutions Advantages
VeloGene Biotech's TurboMice™ Humanize Service Can Cover Complex Scenarios
- Single-gene humanization (CDS / single-exon / multi-exon / full-coding-region replacement)
- Protein domain humanization, point mutation combinatorial humanization
- Dual/triple-gene humanization arrays (immune checkpoints, cytokine clusters)
- Long-fragment humanization over 20 kb (such as HLA clusters, immunoglobulin loci, PCSK9 regions)
VeloGene's Delivery Period(As quick as in 3 months)
- Editing strategy customization: 1–2 days
- Vector construction: 2–4 weeks
- ESC editing + single-clone verification: 3–6 weeks
- Tetraploid complementation mouse Weaning, quality inspection & shipping: Approx. 3 weeks
Homozygous Humanized Mice Delivered in as Fast as 3-5 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
Clients Cases
1. GeneAssist and VeloGene Biotechnology Jointly Announce: Launch of the World's First/Series of Humanized DMD Mouse Models to Empower Global New Drug R&D
DMD is a fatal X-linked recessive genetic disease and one of the most challenging problems in the field of rare diseases. The long-standing lack of animal models that can accurately simulate the pathological characteristics of human patients has been a key bottleneck restricting the clinical translation rate of global DMD drug development.
To address these pain points, GeneAssist, leveraging its deep expertise in gene editing technology and the DMD disease field, has partnered with VeloGene Biotechnology’s globally leading TurboMice™ tetraploid complementation technology platform. Together, they have successfully broken through the technical barriers of complex DMD model construction, developing multiple humanized DMD animal models that highly recapitulate human disease phenotypes. The humanized DMD exon 50, 51, 53, and 45 mouse models cover over 30% of the DMD patient population, making them particularly suitable for the preclinical evaluation of DMD exon-skipping therapies.
2. VeloGene Biotechnology Congratulates Partner AccurEdit Therapeutics on Receiving US FDA IND Clearance for In Vivo Gene Editing Therapy ART001
VeloGene Biotechnology leveraged its high-efficiency TurboMice™ tetraploid complementation technology to rapidly complete the iteration of 2nd-generation new target animal models for AccurEdit Therapeutics in just 4 months. This successfully developed humanized gene editing mouse models carrying rare human genetic disease mutations with ideal phenotypes, which were quickly mass-produced for the preclinical study of ART001, contributing to the acceleration of innovative gene therapy R&D for ATTR diseases.
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Frequent Asks
Humanized mice from scratch traditionally take more than 6 months. Can you really deliver in 3 months?
Traditional method involves vector construction + ES chimerism + germline transmission + homozygous screening; whereas TurboMice™ completes the humanization all at once at the ESC level, with founder mice (F0) being directly homozygous, enabling the delivery of experiment-ready mice within 3 to 5 months.”
Is >20kb long fragment humanizing really possible?
Affirmative. ESC homologous recombination is not limited by pronuclear injection volume, and VeloGene has successfully delivered orders involving humanization and multi-locus combinations of over 20 kb.
Can F0 generation humanized mice be used directly for pharmacodynamic experiments?
Positive. F0 whole-body cells uniformly carry the humanized allele, and protein expression is verified via QC. They can directly enter tumor, immunity, and infection evaluations without the need for further expansion.
What if I want more mice in the future?
Cryopreserved ESC clones are directly revived to edit lines, delivering live animals in batches within 2 months, featuring identical strain and origin with zero genetic drift.