The Next Generation of Mouse Model Preparation Platform
- 4-Week Delivery: World’s fastest custom mouse model development.
- TurboMice™ Tech: Proprietary high-efficiency gene-editing platform.
- Full Capabilities: Expert conditional knockouts, knock-ins, and humanized models.
- Validated Quality: Guaranteed high precision and model viability.
VeloGene Biotechnology
Mouse Customization - Conditional Knockout(cKO) Service
F0 Cre⁺fl/fl Ready - to -Use
VeloGene Biotechnology Point Mutation Mouse Model Customizing Service
F0 "Founder" Ready - to -Use
Point Mutation Mice refer to genetically modified models in which single nucleotide variations (SNVs), small insertions/deletions (InDel), or missense/nonsense mutations are introduced into specific genomic sites of mice using gene-editing technology, thereby precisely simulating human genetic disease-causing loci or specific protein function alterations.
VeloGene Biotechnology’s TurboMice™ tetraploid complementation technology completes in situ point mutation editing at the embryonic stem cell (ESC) level, followed by aggregation with tetraploid embryos (which form only extraembryonic tissues such as the placenta) to compensate development. Therefore, all F0 generation mice originate from the same edited ESC monoclonal line, allowing the delivery of homozygous and stably heritable point mutation mice within 3 to 5 months without the need for multi-generation breeding and screening.
Traditon Point Mutation vs TurboMice™ Point Mutation
| Metrics | Traditional ES Chimerism / Pronuclear Injection / CRISPR | VeloGene TurboMice™ Point Mutation Customization |
|---|---|---|
| Time to Obtain Homozygous Usable Point Mutation Mice | 6–12 months (multi-generation intercrossing & screening) | Delivery in 3–5 months |
| Precise Single Base Substitution (SNV) | Prone to random InDel generation, homozygous screening is difficult | ESC homologous recombination, precise introduction of target mutations |
| Same-Gene Multi-Site Mutation Combinations | Linkage drift, out-of-control breeding cycles | One-time multi-mutation accumulation via ESC monoclonal lines |
| F0 Direct Experimental Usability | Mostly chimeric / heterozygous, requires expansion validation | Yes, F0 mice are directly homozygous with full genotypes |
| Genetic Background Consistency | Prone to genetic drift through multi-generation intercrossing | Clone-level uniformity via same-strain ESCs |
| Cryopreservation & Expansion | Sperm/embryo cryopreservation, recovery & breeding takes 1 year+ | ESC cryopreservation, batch delivery within 2 months |
VeloGene Biotechnology's Point Mutaion Mouse Solutions Advantages
VeloGene Biotech's TurboMice™ cKO Service Can Cover Complex Scenarios
- Single-gene conditional knockout (Cre⁺ / Flox⁻/⁻)
- Single-Chromosome Multiplex Editing for Co-delivery of Floxed Sites, Cre Cassette, and Reporter Gene
- Dual-gene / triple-gene conditional knockouts (multi-loxP arrays)
- Large-fragment humanization (>20 kb) + flox combination · Inducible CKO (CreERT2) + site-specific point mutations
- Specific strain backgrounds: C57BL/6, BALB/c, etc.
VeloGene's Delivery Period(As quick as in 4 months)
- Editing Strategy Customization: 1–2 days
- Vector Construction: 4–6 weeks
- ESC Editing + Monoclonal Screening & Validation: 4–8 weeks
- Tetraploid Complementation Mouse Production: 4–8 weeks
- Mouse Weaning, Quality Control & Shipping: Approx. 4 weeks
VeloGene Biotechnology's Specialties
- Zero Genetic Drift: Same-batch homologous ESC clones ensure extremely low individual variability
- Ready-to-Use F0: No longer hindered by chimera rates or germline transmission loss
- Reproducible Complex Genotypes: Multi-locus combinations on the same chromosome no longer rely on “luck-based breeding”
- Ultra-Fast Batch Delivery: Initial CKO founder mice delivered in 4–6 months, with subsequent cohort expansion delivered in batches within 2 months
Cre⁺/Flox Homozygous CKO Mice Delivered in as Fast as 4–6 Months (Rapid batch generation from ES cells to mice takes approximately 45 days at the fastest)
Order Now to Receive 2 Years of Free ESC Cryopreservation/Banking (No need for re-mating for subsequent cohort expansion; batch delivery in approximately 2 months)
How to get your mice?
Send Your Inquiry
Before we start, you can start by submitting your inquiry form or talk to us on our Whatsapp and tell us what you need!
Solution Design
We will reply to you as soon as possible and send you a quote!
TurboMicing
We will get hands on your project.
Get your Ready to Use f0 Mice!
Our f0 homozygous mice will ship to you and lab-ready!
Frequent Asks
Conditional knockout mice traditionally take a year. Can you really deliver in 4 months?
“Traditional breeding involves generating Flox mice + introducing Cre mice + double-heterozygous mating + homozygous screening; TurboMice™ simultaneously integrates flox and cre elements at the ESC stage and directly produces F0 homozygotes via tetraploid complementation, enabling experimental-ready mice delivery in 4–6 months.”
Can two loci on the same chromosome be floxed together?
Yes. Multi-round editing at the ESC level is not limited by meiotic linkage, and VeloGene has delivered dual/triple-target and over-100-kb fragment editing orders.
Can F0-generation CKO mice be directly used for tissue-specific phenotypic assays?
Yes. F0 cells uniformly carry Cre⁺ and flox⁻/⁻ across the entire body, allowing tissue-specific recombination to be triggered via inducers or endogenous promoters without further breeding.
How are subsequent mouse orders handled?
Cryopreserved ESCs are thawed for a batch delivery in 2 months, maintaining a consistent genetic background.
IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.