hCD28 Mouse Model
| Strain Name | hCD28 Mouse Model |
|---|---|
| Strain Background | C57BL/6N |
Applications
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Anti-CD28 antibody evaluation: Test agonist/antagonist mAbs for safety and PD readouts
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Bispecific TCE testing: Validate CD3×CD28 engagers requiring human CD28 co-stimulation
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T cell activation studies: Dissect CD28–PI3K–AKT and PKCθ–NF-κB signaling in vivo
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Autoimmunity & checkpoint research: Model CD28-dependent T effector vs Treg balance
Strain Description
The hCD28 Mouse Model carries a targeted knock-in of the human CD28 gene at the endogenous mouse locus. It replaces the murine Cd28 extracellular coding sequence while preserving native promoters and regulatory elements. Therefore, it ensures physiological expression of functional human CD28 protein in the same cell types where the endogenous gene is normally expressed, predominantly T cells.
In this hCD28 model, CD28 engagement delivers a critical second signal alongside TCR activation. Its cytoplasmic YMNM motif recruits PI3K to activate AKT-mTOR, promoting glucose uptake and glycolytic reprogramming. The PYAP motif recruits LCK and PKCθ, which trigger NF-κB, NFAT, and AP-1 to drive IL-2 production and clonal expansion. Furthermore, CD28 signaling supports regulatory T cell homeostasis and memory CD8+ T cell formation.
This humanized CD28 model allows therapeutics to engage authentic human CD28 epitopes. For example, you can test agonist or antagonist anti-CD28 monoclonal antibodies for safety and pharmacodynamics. Similarly, it supports evaluation of CD3×CD28 bispecific T cell engagers (TCEs) that require human CD28 for T cell activation. Consequently, the hCD28 Mouse Model provides a translational platform for immuno-oncology drug efficacy and co-stimulation modulation that conventional models cannot replicate.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
How much for a project assessment?
•Free initial design proposal with zero obligations.
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Who owns the mouse lP? Do l need a licence from Mingceler?
All model generation projects of Mingceler operate under a fee-for-service framework.
IP Ownership: All intellectual property rights related to custom mouse models, including derived organs, tissues, cells, and biological materials, are the sole and exclusive property of the Client.
Third-Party Transfer Permission: The Client may independently decide to retain, utilize, or commercialize their custom models project materials (e.g., targeting vectors, ES cells, mouse lines) without the need for prior consent from Mingceler.
Licensing Exemption: The Client has full autonomy over all uses of the custom models or their derivatives, including but not limited to commercialization, distribution to third parties, and publication involving model data. No written license from Mingceler is required for such uses.