Why VeloGene?
Cell Services
Feeder Cells & MEF Cells
VeloGene Feeder Cells
Why Choose Our Feeders?
1. Save Time & Resources: Avoid the hassle of primary cell isolation and inactivation.
2. Reduce Variability: Achieve more reproducible results with our highly consistent batches.
3. Expert Support: Backed by our team's extensive experience in cell culture applications.
VeloGene's Ready-to-Use Feeder Cells for Robust Stem Cell CultureEliminate the inconsistency and labor-intensive process of preparing your own feeder layers. Our pre-tested, mitomycin-C-treated or gamma-irradiated feeder cells provide a reliable foundation for your most sensitive cultures. They are designed to secrete necessary nutrients and create an optimal microenvironment, maximizing plating efficiency and supporting long-term stem cell growth while minimizing spontaneous differentiation.
Product Features
High Nourishing Capacity
Pre-inactivated Treatment
Rich Selection of Resistance Markers
High Post-Thaw Viability
Feeder Cell Preparation Process
Have you ever wondered how these remarkable feeder cells come to play a pivotal role in your scientific research? The journey of creating these essential cells is a meticulous scientific odyssey.
It all begins with the careful extraction of embryonic tissue from pregnant mice precisely at 13.5 days post - coitum. Through a sequence of painstaking separation and purification procedures, we isolate Primary Mouse Embryonic Fibroblasts (MEF cells), marking the starting point as passage 0 (P0).
These nascent P0 MEF cells then embark on a growth journey, undergoing expansion through successive passages. To ensure they serve their intended function without uncontrolled growth, we treat the expanded cells with Mitomycin C. This treatment effectively reins in their proliferative capacity, transforming them into the highly - valued feeder cells.
Once the feeder cells are ready, they are cryopreserved and securely stored in liquid nitrogen tanks, safeguarded for long - term use. Quality is the cornerstone of this process. Throughout the entire preparation, we implement rigorous quality control measures. We closely examine cell morphology to ensure the cells maintain their proper structure, conduct viability tests to confirm their health and functionality, and carry out mycoplasma detection to keep contamination at bay.
For researchers in stem cell research, tissue engineering, or any field where feeder cells are integral, understanding this preparation process is not just academic; it's the key to unlocking the full potential of these cells in your experiments. Whether you're aiming to support stem cell growth, study cell - cell interactions, or develop new therapeutic strategies, these feeder cells, crafted with precision, are your reliable partners.
VeloGene MEF Cells
VeloGene Cell Products' Portfolio
| Brand | Starin | Cell Amount | Treatment | Features | Type | Availability |
|---|---|---|---|---|---|---|
| VeloGene/MingCeler | C572 | million | None | 4-drug resistance (Puro, Neo, Hygro, Blast) | MEF | In Stock |
| VeloGene/MingCeler | C57 | 2 miliion | MitoC | 4-drug resistance (Puro, Neo, Hygro, Blast) | Feeder | Inquiry |
| VeloGene/MingCeler | ICR | 2 miliion | None | None | MEF | In Stock |
| VeloGene/MingCeler | CF-1 | 2 miliion | None | None | MEF | In Stock |
| VeloGene/MingCeler | 129/0G2 | 2 million | None | iPSC reprogramming | MEF | Inquiry |
| VeloGene/MingCeler | ICR | 2 million | MitoC | MitoC | Feeder | In Stock |
| VeloGene/MingCeler | CF-1 | 2 million | MitoC | MitoC | Feeder | In Stock |
IP Assurance
At VeloGene, we operate on a strict fee-for-service model. All intellectual property (IP) and materials created for your project are 100% yours. We take every measure to safeguard your IP, ensuring no vectors, ES cells, or mouse lines are retained or resold to third parties. This way, you can focus on your research with complete peace of mind.