nuNPG Mouse Model
| Starin Name | NOD-Prkdc<sup>tm1Ming</sup>Il2rg<sup>tm1Ming</sup>/MC |
|---|---|
| Strain Background | NOD/ShiLtJ |
Applications
- Xenograft Tumor Research: CDX and PDX model establishment for anticancer drug screening and efficacy assessment.
- Humanized Mouse Model Development: Engraftment of human CD34⁺ hematopoietic stem cells (HSC) or PBMCs for immune system reconstitution.
- Stem Cell Research: Supporting the study of human ES/iPS cells and hematopoietic differentiation in vivo.
- Infectious Disease & Vaccine Research: Modeling human-tropic pathogen infection in a humanized immune background.
- Immuno-oncology & Hematopoiesis: Investigation of human immune cell development, function, and tumor–immune interactions.
Key Features
- Complete Lymphocyte Deficiency: Absence of mature T, B, and NK cells in peripheral blood; no B-cell leakage.
- No Prkdc “Leakage”: Deletion of Prkdcexons 2–10 eliminates age-related leaky T/B cell reconstitution seen in conventional Prkdcscidmice.
- High Engraftment Efficiency: NOD background suppresses innate phagocytic activity, enabling robust human cell/tissue acceptance with minimal cell numbers required for tumor take.
- Validated Phenotype: Confirmed absence of T/B/NK cells by flow cytometry per MINGCELER GENETIC POLICY®.
Strain Description
nuNPG™ Severe Combined Immunodeficient (NOD-Prkdctm1MingIl2rgtm1Ming) Mouse Model
A triple-immunodeficient mouse on the NOD inbred background with homozygous knockout of Prkdc(exons 2–10) and Il2rg(exons 3–6), resulting in the complete absence of functional T, B, and NK cells. This model supports high-efficiency engraftment of human cells, tissues, and patient-derived xenografts (PDX/CDX), making it an optimal platform for humanized mouse studies and oncology drug evaluation.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
How much for a project assessment?
•Free initial design proposal with zero obligations.
•Request a free quote!
Who owns the mouse lP? Do l need a licence from Mingceler?
All model generation projects of Mingceler operate under a fee-for-service framework.
IP Ownership: All intellectual property rights related to custom mouse models, including derived organs, tissues, cells, and biological materials, are the sole and exclusive property of the Client.
Third-Party Transfer Permission: The Client may independently decide to retain, utilize, or commercialize their custom models project materials (e.g., targeting vectors, ES cells, mouse lines) without the need for prior consent from Mingceler.
Licensing Exemption: The Client has full autonomy over all uses of the custom models or their derivatives, including but not limited to commercialization, distribution to third parties, and publication involving model data. No written license from Mingceler is required for such uses.