The Next Generation of Mouse Model Preparation Platform
Why VeloGene?
TurboMice™ - Rapid Mouse Model Technology
Mono Chromosome Multipoint Gene Editing
From Cell to Homozygous Mice in 45 Days
VeloGene TurboMice™ Tetraploid Complementation Technology: Overcoming Multi-Loci Editing on the Same Chromosome, Delivering "Clone-Grade" Mice Directly at the F0 Generation
In the construction of genetically engineered animal models, researchers often face a challenging bottleneck: multi-loci editing on the same chromosome.Especially when two or more editing sites are located on the same chromosome, traditional technical routes are virtually impassable. Even if attempted, the subsequent prolonged breeding and screening process is often accompanied by uncontrollable genetic drift, significantly undermining the stability and reproducibility of experimental data.How to break the deadlock? VeloGene TurboMice™ Tetraploid Complementation Technology is emerging as a powerful tool to conquer this industry pain point.
1. The Dilemma of the Traditional Path: Lengthy Waits and "Schrödinger’s" Genes
Traditional pronuclear injection or ES cell chimerism technologies follow a “mice-breeding-mice” pathway. This path is not only long, but also full of variables:
Prolonged Timeline: Requires multiple generations of mating and screening, easily taking six months to a year or more.
Random Transmission: Germline transmission in chimeric mice exhibits significant randomness. Whether the target gene is passed down and to what extent is like opening a blind box.
Background Drift: Genetic drift is extremely prone to occur during multi-generation propagation, leading to fluctuations in experimental data and making individual consistency difficult to guarantee.
Multi-Loci Editing on the Same Chromosome: Almost impossible to achieve.
This is undoubtedly a major constraint for modern life science research that pursues precision and efficiency.
2. TurboMice™ Tetraploid Complementation Technology: Underlying Logic Reconstruction from "Breeding & Screening" to "Source Customization"
The emergence of TurboMice™ Tetraploid Complementation Technology reconstructs the production process of gene-edited animals from its underlying logic.Its core principle is straightforward yet revolutionary: diploid embryonic stem cells that have undergone precise multiple editing are injected into tetraploid embryos that can only develop into extraembryonic tissues such as the placenta.The resulting F0 generation mice have their entire somatic tissue genetic material derived 100% from that precisely edited embryonic stem cell clone.
What does this mean?
No Breeding Required: Skips the lengthy multi-generation screening stage; the F0 generation is directly fully genotyped mice.
Uniform Background: All mice originate from the same stem cell clone, ensuring a highly consistent genetic background and completely eliminating data noise caused by individual differences.
Breaking Limitations: Easily achieves simultaneous editing of multiple sites on the same chromosome.
This does not violate natural laws; rather, it is a more efficient, controllable, and reproducible scientific research pathway forged through a deep understanding of developmental biology.
From Laboratory to Industrialization: The "China Speed" of 30%-60% Live Birth Rates
A cutting-edge technology can truly empower scientific research only when it steps out of the laboratory and achieves stable, efficient industrialization.
Dr. Guoming Wu, founder of VeloGene, has been deeply dedicated to the field of embryonic development for over thirty years. He led his team to optimize the tetraploid complementation technology across the entire workflow, breaking through multiple practical bottlenecks.
Hard-core data is the best proof:
Industry Average Level: The embryo live birth rate for tetraploid technology is typically only 1%-5%.
VeloGene Level: Across multiple strains and model constructions, a stable live birth rate of 30%-60% is achieved, with efficiency approaching conventional embryo transfer experiments.
A dozen-fold increase in efficiency directly solves the most critical pain points of cost and success rate in industrialization. To date, VeloGene has successfully delivered nearly 300 custom tetraploid complementation technology model orders, covering single-target, dual-target, triple-target, and even hundreds of kb large-fragment editing, fully verifying the stability and universality of the technology.
VeloGene TurboMice™ Tetraploid Complementation Technology makes complex gene editing simple, achieving multi-loci editing on the same chromosome in a single step. Researchers and partners are welcome to consult with us.