hTFRC Mouse Model
| Starin Name | C57BL/6N‑Tfrc<sup>tm1(hTFRC)</sup> |
|---|---|
| Strain Background | C57BL/6N |
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Applications
- BBB Shuttle Candidate Screening: In vivo evaluation of TFRC-directed bispecific antibodies or fusion proteins for blood–brain barrier transcytosis and brain parenchyma exposure.
- Molecular Engineering Parameter Assessment: Determining the impact of antibody affinity, valency, and binding epitope on endosomal sorting fate (transcytosis vs. recycling vs. lysosomal degradation).
- Dose–Response & PK/PD Optimization: Establishing dose ranges and receptor occupancy dynamics for TFRC-targeted therapeutics under human-relevant competition from endogenous transferrin.
- Iron Metabolism & Erythropoiesis Research: Investigating human TFRC function in cellular iron uptake and red blood cell development (validated high expression on erythroblasts/bone marrow RBCs).
- Immuno-toxicity & Biodistribution Profiling: Assessing off-target tissue binding and immune cell subset distribution in a human TFRC-dominant background.
Key Features
- Targeted Extracellular Domain Humanization: Murine TfrcECD replaced with human TFRCsequence via knock-in; TM & cytoplasmic tail retained to preserve physiological recycling/signaling.
- No Mixed-Dimer Interference: Homozygous humanization eliminates aberrant human–mouse TFRC heterodimerization, ensuring data reflect purely human TFRC–mediated transcytosis.
- Physiological Expression & Immune Homeostasis: Human TFRC highly expressed in liver, brain endothelium, and bone marrow erythroblasts; splenic T/B/NK cell proportions indistinguishable from WT.
- Translational Relevance for BBB Therapeutics: Overcomes species-specific epitope/affinity differences of anti-human TFRC antibodies, enabling direct preclinical validation of BBB-shuttle modalities.
Strain Description
hTFRC Humanized Knock-in Mouse Model (C57BL/6N‑Tfrctm1(hTFRC)/MCL)
This model replaces the extracellular domain of the endogenous murine Tfrcgene with the corresponding human TFRCsequence on the C57BL/6N background, while preserving the mouse transmembrane and intracellular domains. It expresses a chimeric TFRC protein bearing human-specific epitopes and functional domains, eliminating species-specific binding discrepancies and mixed-dimer interference. This model provides a physiologically relevant in vivoplatform for evaluating TFRC-targeted Bispecific Antibodies (BsAbs), Antibody–Drug Conjugates (ADCs), and BBB-shuttle modalities for brain delivery.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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Who owns the mouse lP? Do l need a licence from Mingceler?
All model generation projects of Mingceler operate under a fee-for-service framework.
IP Ownership: All intellectual property rights related to custom mouse models, including derived organs, tissues, cells, and biological materials, are the sole and exclusive property of the Client.
Third-Party Transfer Permission: The Client may independently decide to retain, utilize, or commercialize their custom models project materials (e.g., targeting vectors, ES cells, mouse lines) without the need for prior consent from Mingceler.
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