hOX40/hOX40L Mouse Model
| Strain Name | hOX40/hOX40L Mouse Model |
|---|---|
| Strain Background | C57BL/6N |
Applications
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Anti-OX40 agonist evaluation: Test OX40-triggering mAbs for tumor-infiltrating lymphocyte expansion and memory persistence
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Anti-OX40L blocker testing: Evaluate amlitelimab-like mAbs in atopic dermatitis and GVHD models
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T cell co-stimulation studies: Dissect OX40–NF-κB/PI3K–AKT signaling in CD4⁺/CD8⁺ T cell activation
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IO combination regimens: Model OX40 agonism combined with PD-1/PD-L1 or CTLA-4 blockade
Strain Description
The hOX40/hOX40L Mouse Model carries targeted knock-in of human OX40 (TNFRSF4) and/or OX40L (TNFSF4) at their endogenous mouse loci. It replaces the murine extracellular coding sequences while preserving native promoters, enhancers, and regulatory elements. Therefore, it ensures physiological expression of functional human OX40 on activated T cells and human OX40L on antigen-presenting cells. This human OX40 OX40L knock-in mouse recreates the authentic ligand–receptor interface in a live animal.
In this hOX40/hOX40L model, OX40 engagement delivers a potent co-stimulatory signal alongside TCR activation. It recruits TRAF2/TRAF5 to activate NF-κB and PI3K–AKT pathways, promoting T cell proliferation, IL-2 production, and long-lived memory formation. Conversely, OX40L ligation on dendritic cells can provide reverse signaling that modulates cytokine secretion profiles. Dysregulated OX40 signaling enhances anti-tumor immunity, whereas persistent OX40L co-stimulation can exacerbate T cell–mediated inflammatory diseases such as atopic dermatitis.
This humanized OX40 OX40L model allows therapeutics to engage authentic human epitopes. For example, you can test OX40-agonist monoclonal antibodies designed to boost anti-tumor T cell responses. Similarly, it supports evaluation of OX40L-blocking antibodies such as amlitelimab analogs for atopic dermatitis and GVHD. Consequently, the hOX40/hOX40L Mouse Model provides a translational platform for immuno-oncology and anti-inflammatory drug efficacy that conventional models cannot replicate.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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