hFAP Mouse Model
| Strain Name | hFAP Mouse Model |
|---|---|
| Strain Background | C57bl/6N |
Applications
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FAP-targeted drug testing: Evaluate radiolabeled FAP inhibitors, anti-FAP mAbs, ADCs, and CAR-T cells.
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Tumor stroma & CAF biology: Study FAP+ CAF roles in ECM remodeling and immune exclusion.
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Immuno-oncology combinations: Model stromal FAP impact on PD-1/PD-L1 blockade response.
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FAP enzymology: Investigate DPPIV-like and endopeptidase activities in a human context.
Strain Description
The hFAP Mouse Model carries a targeted knock-in of the human FAP gene at the endogenous mouse locus. It replaces the murine Fap coding sequence while preserving native promoters and regulatory elements. Therefore, it ensures physiological expression of functional human fibroblast activation protein (FAP) in the same cell types where the endogenous gene is normally expressed, primarily activated fibroblasts and cancer-associated fibroblasts (CAFs). This human FAP knock-in model produces human FAP that functions in extracellular matrix remodeling and tumor stroma biology in a physiologically relevant manner.
FAP is a type II transmembrane serine protease almost exclusively expressed by cancer-associated fibroblasts (CAFs). It remodels the extracellular matrix via dipeptidyl peptidase and endopeptidase activities. Furthermore, FAP+ CAFs secrete chemokines such as CXCL12 to shape an immunosuppressive tumor microenvironment. For example, they recruit MDSCs and block cytotoxic T-cell infiltration, driving resistance to checkpoint inhibition.
This humanized FAP model allows therapeutic agents such as anti-FAP antibodies, CAR-T cells, or radiolabeled peptides to bind human FAP in vivo. Consequently, it provides a translational platform for evaluating FAP-targeted drugs, studying CAF-mediated immune exclusion, and modeling human stromal biology that conventional models cannot replicate.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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Who owns the mouse lP? Do l need a licence from Mingceler?
All model generation projects of Mingceler operate under a fee-for-service framework.
IP Ownership: All intellectual property rights related to custom mouse models, including derived organs, tissues, cells, and biological materials, are the sole and exclusive property of the Client.
Third-Party Transfer Permission: The Client may independently decide to retain, utilize, or commercialize their custom models project materials (e.g., targeting vectors, ES cells, mouse lines) without the need for prior consent from Mingceler.
Licensing Exemption: The Client has full autonomy over all uses of the custom models or their derivatives, including but not limited to commercialization, distribution to third parties, and publication involving model data. No written license from Mingceler is required for such uses.