Pdx1-IRES-iCre Mouse Model
| Starin Name | C57BL/6N‑Pdx1<sup>tm1(IRES‑iCre)</sup> |
|---|---|
| Strain Background | C57BL/6N |
Applications
- Pancreas Development Research: Studying pancreatic bud formation, specification of endocrine/exocrine lineages, and the role of Pdx1 in embryogenesis.
- Diabetes & Metabolic Disease Models: Conditional knockout of candidate genes in β-cells to investigate type 1/type 2 diabetes pathogenesis and β-cell function/maintenance.
- Cell Lineage Tracing: Crossing with Cre-dependent reporter strains (e.g., mT/mG, Rosa26tdTomato) to track Pdx1-positive cell fate during development and adult tissue homeostasis.
- Pancreatitis & Pancreatic Disease Mechanisms: Tissue-specific gene manipulation to model pancreatitis, neoplasia, or δ-cell biology.
- Conditional Transgenic Validation: Facilitating β-cell–restricted or pancreas–restricted gain/loss-of-function studies in vivo.
Key Features
- Bicistronic Co-expression via IRES: iCre is expressed from the endogenous Pdx1locus using an IRES element, ensuring identical spatiotemporal expression patterns to native Pdx1 protein.
- Preserved Pdx1 Function: Precise insertion after the stop codon leaves Pdx1open reading frame and protein function intact—normal pancreatic development and glucose homeostasis are maintained.
- High Lineage Specificity: Cre activity restricted to Pdx1-positive cells (pancreatic β-cells, δ-cells, pancreatic progenitors, duodenal enteroendocrine cells), with no ectopic recombination in non-target tissues (e.g., heart).
- Clean Conditional Recombination: Enables floxed allele excision exclusively in Pdx1 lineage, avoiding confounding phenotypes from whole-body or early embryonic gene deletion.
Strain Description
Pdx1-IRES-iCre Knock-in Mouse Model (C57BL/6N-Pdx1tm1(IRES-iCre)/MCL)
This model is generated by inserting an IRES-iCre cassette immediately after the stop codon of the endogenous Pdx1gene (before the 3′UTR) on the C57BL/6N background. Pdx1and improved Cre recombinase (iCre) are bicistronically expressed under the native Pdx1promoter, enabling Cre-mediated conditional gene recombination specifically in Pdx1-lineage cells (pancreatic progenitors, β-cells, duodenal endocrine cells) without disrupting endogenous Pdx1 function.
FAQ
Game-changing benefits？
While competitors highlight germline efficiency gains, shorter timelines and enhanced 3Rs animal welfare benefits for their technologies, these are merely incremental improvements over traditional approaches. In sharp contrast, our proprietary technology delivers fully pure, homogeneous lineages—every single cell of the mice is derived exclusively from totipotent ES cells, with guaranteed 100% germline transmission efficiency. To experience these unparalleled benefits firsthand, enquire about your custom mouse model project with us or order embryos for in-house validation at your facility.
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